제품 > 항체 > 다중클론항체(pAb)

CPA1 Rabbit pAb (A3803)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CPA1 Rabbit pAb (A3803)

Western blot analysis of extracts of various cell lines, using CPA1 antibody (A3803) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - CPA1 Rabbit pAb (A3803)

Immunofluorescence analysis of human pancreas cells using CPA1 Rabbit pAb (A3803) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCPA1 Rabbit pAb
Catalog No.A3803
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CPA1 (NP_001859.1).
SequenceMRGLLVLSVLLGAVFGKEDFVGHQVLRISVADEAQVQKVKELEDLEHLQLDFWRGPAHPGSPIDVRVPFPSIQAVKIFLESHGISYETMIEDVQSLLDEE
Gene ID
Swiss Prot
SynonymsCPA
Calculated MW47kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse pancreas, Mouse spleen, Mouse brain, Rat pancreas
Cellular locationSecreted

Documents

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ABclonal:Western blot - CPA1 Rabbit pAb (A3803)}

Western blot - CPA1 Rabbit pAb (A3803)

Western blot analysis of extracts of various cell lines, using CPA1 antibody (A3803) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - CPA1 Rabbit pAb (A3803)}

Immunofluorescence - CPA1 Rabbit pAb (A3803)

Immunofluorescence analysis of human pancreas cells using CPA1 Rabbit pAb (A3803) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.