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CPT2 Rabbit pAb (A12426)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CPT2 Rabbit pAb (A12426)

Western blot analysis of various lysates using CPT2 Rabbit pAb (A12426) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - CPT2 Rabbit pAb (A12426)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CPT2 Rabbit pAb (A12426) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CPT2 Rabbit pAb (A12426)

Immunofluorescence analysis of NIH/3T3 cells using CPT2 Rabbit pAb (A12426) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CPT2 Rabbit pAb (A12426)

Immunofluorescence analysis of PC-12 cells using CPT2 Rabbit pAb (A12426) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCPT2 Rabbit pAb
Catalog No.A12426
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 404-658 of human CPT2 (NP_000089.1).
SequenceATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS
Gene ID
Swiss Prot
SynonymsCPT1; IIAE4; CPTASE; CPT2
Calculated MW74kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, HepG2, Mouse liver, Rat liver
Cellular locationMatrix side, Mitochondrion inner membrane, Peripheral membrane protein.
Customer validation

WB(Mus musculus, Gallus gallus)

Documents

Certificate of Compliance

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ABclonal:Western blot - CPT2 Rabbit pAb (A12426)}

Western blot - CPT2 Rabbit pAb (A12426)

Western blot analysis of various lysates using CPT2 Rabbit pAb (A12426) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - CPT2 Rabbit pAb (A12426)}

Immunohistochemistry - CPT2 Rabbit pAb (A12426)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CPT2 Rabbit pAb (A12426) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CPT2 Rabbit pAb (A12426)}

Immunofluorescence - CPT2 Rabbit pAb (A12426)

Immunofluorescence analysis of NIH/3T3 cells using CPT2 Rabbit pAb (A12426) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CPT2 Rabbit pAb (A12426)}

Immunofluorescence - CPT2 Rabbit pAb (A12426)

Immunofluorescence analysis of PC-12 cells using CPT2 Rabbit pAb (A12426) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12426? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)