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CPT2 Rabbit pAb (A2876)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CPT2 Rabbit pAb (A2876)

Western blot analysis of lysates from Rat liver, using CPT2 Rabbit pAb (A2876) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CPT2 Rabbit pAb (A2876)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using CPT2 Rabbit pAb (A2876) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CPT2 Rabbit pAb (A2876)

Immunohistochemistry analysis of paraffin-embedded rat brain using CPT2 Rabbit pAb (A2876) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameCPT2 Rabbit pAb
Catalog No.A2876
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 420-658 of human CPT2 (NP_000089.1).
SequenceTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS
Gene ID
Swiss Prot
SynonymsCPT1; IIAE4; CPTASE; CPT2
Calculated MW74kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat liver
Cellular locationMatrix side, Mitochondrion inner membrane, Peripheral membrane protein

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ABclonal:Western blot - CPT2 Rabbit pAb (A2876)}

Western blot - CPT2 Rabbit pAb (A2876)

Western blot analysis of lysates from Rat liver, using CPT2 Rabbit pAb (A2876) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CPT2 Rabbit pAb (A2876)}

Immunohistochemistry - CPT2 Rabbit pAb (A2876)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using CPT2 Rabbit pAb (A2876) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CPT2 Rabbit pAb (A2876)}

Immunohistochemistry - CPT2 Rabbit pAb (A2876)

Immunohistochemistry analysis of paraffin-embedded rat brain using CPT2 Rabbit pAb (A2876) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (24)