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CRALBP Rabbit mAb (A9265)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CRALBP Rabbit mAb (A9265)

Western blot analysis of lysates from Mouse eye, using CRALBP Rabbit mAb (A9265) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - CRALBP Rabbit mAb (A9265)

Immunohistochemistry analysis of paraffin-embedded Rat retina using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CRALBP Rabbit mAb (A9265)

Immunohistochemistry analysis of paraffin-embedded Mouse retina using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - CRALBP Rabbit mAb (A9265)

Immunofluorescence analysis of paraffin-embedded rat eye using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CRALBP Rabbit mAb (A9265)

Immunofluorescence analysis of paraffin-embedded mouse eye using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCRALBP Rabbit mAb
Catalog No.A9265
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1502
The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CRALBP (P12271).
SequenceMSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFI
Gene ID
Swiss Prot
SynonymsCRALBP
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:100 - 1:1000
  • IF/ICC 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse eye
Cellular locationCytoplasm.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CRALBP Rabbit mAb (A9265)}

    Western blot - CRALBP Rabbit mAb (A9265)

    Western blot analysis of lysates from Mouse eye, using CRALBP Rabbit mAb (A9265) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - CRALBP Rabbit mAb (A9265)}

    Immunohistochemistry - CRALBP Rabbit mAb (A9265)

    Immunohistochemistry analysis of paraffin-embedded Rat retina using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - CRALBP Rabbit mAb (A9265)}

    Immunohistochemistry - CRALBP Rabbit mAb (A9265)

    Immunohistochemistry analysis of paraffin-embedded Mouse retina using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - CRALBP Rabbit mAb (A9265)}

    Immunofluorescence - CRALBP Rabbit mAb (A9265)

    Immunofluorescence analysis of paraffin-embedded rat eye using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - CRALBP Rabbit mAb (A9265)}

    Immunofluorescence - CRALBP Rabbit mAb (A9265)

    Immunofluorescence analysis of paraffin-embedded mouse eye using CRALBP Rabbit mAb (A9265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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