제품 > 항체 > 다중클론항체(pAb)

CRISP2 Rabbit pAb (A7177)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - CRISP2 Rabbit pAb (A7177)

Western blot analysis of extracts of various cell lines, using CRISP2 antibody (A7177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.

Overview

Product nameCRISP2 Rabbit pAb
Catalog No.A7177
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in cell-cell adhesion. Predicted to be active in extracellular space.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-243 of human CRISP2 (NP_003287.1).
SequenceKDPAFTALLTTQLQVQREIVNKHNELRKAVSPPASNMLKMEWSREVTTNAQRWANKCTLQHSDPEDRKTSTRCGENLYMSSDPTSWSSAIQSWYDEILDFVYGVGPKSPNAVVGHYTQLVWYSTYQVGCGIAYCPNQDSLKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDDCDKGLCTNSCQYQDLLSNCDSLKNTAGCEHELLKEKCKATCLCENKIY
Gene ID
Swiss Prot
SynonymsCT36; TPX1; TSP1; GAPDL5; CRISP-2
Calculated MW27kDa
Observed MW27kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse testis, Mouse liver, Rat liver
Cellular locationSecreted

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    ABclonal:Western blot - CRISP2 Rabbit pAb (A7177)}

    Western blot - CRISP2 Rabbit pAb (A7177)

    Western blot analysis of extracts of various cell lines, using CRISP2 antibody (A7177) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.