제품 > 항체 > 다중클론항체(pAb)

CRTC1 Rabbit pAb (A15412)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - CRTC1 Rabbit pAb (A15412)

Western blot analysis of lysates from Mouse brain, using CRTC1 Rabbit pAb (A15412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameCRTC1 Rabbit pAb
Catalog No.A15412
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cAMP response element binding protein binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in cytosol; nuclear body; and plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CRTC1 (NP_056136.2).
SequenceMATSNNPRKFSEKIALHNQKQAEETAAFEEVMKDLSLTRAARLQLQKSQYLQLGPSRGQYYGGSLPNVNQIGSGTMDLPFQTPFQSSGLDTSRTTRHHGLVDRVYRERGRLGSPHRRPLSVDKHGRQADSCPYGTMYLSPPADTSWRRTNSDSALHQSTMTPTQPESFSSGSQDVHQKRVLLLTVPGMEETTSEADKNLS
Gene ID
Swiss Prot
SynonymsMAML2; MECT1; Mam-2; TORC1; TORC-1; WAMTP1; CRTC1
Calculated MW67kDa
Observed MW80kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationCytoplasm, Nucleus

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ABclonal:Western blot - CRTC1 Rabbit pAb (A15412)}

Western blot - CRTC1 Rabbit pAb (A15412)

Western blot analysis of lysates from Mouse brain, using CRTC1 Rabbit pAb (A15412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.