제품 > 항체 > 다중클론항체(pAb)

CRYGC Rabbit pAb (A9324)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CRYGC Rabbit pAb (A9324)

Western blot analysis of extracts of various cell lines, using CRYGC antibody (A9324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - CRYGC Rabbit pAb (A9324)

Immunofluorescence analysis of L929 cells using CRYGC antibody (A9324) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CRYGC Rabbit pAb (A9324)

Immunofluorescence analysis of C6 cells using CRYGC antibody (A9324) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCRYGC Rabbit pAb
Catalog No.A9324
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the beta/gamma-crystallin family of proteins. Crystallins constitute the major proteins of vertebrate eye lens and maintain the transparency and refractive index of the lens. This gene and several family members are present in a gene cluster on chromosome 2. Mutations in this gene have been shown to cause multiple types of cataract, including Coppock-like cataract and zonular pulverulent cataract, among others.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-174 of human CRYGC (NP_066269.1).
SequenceMGKITFYEDRAFQGRSYETTTDCPNLQPYFSRCNSIRVESGCWMLYERPNYQGQQYLLRRGEYPDYQQWMGLSDSIRSCCLIPQTVSHRLRLYEREDHKGLMMELSEDCPSIQDRFHLSEIRSLHVLEGCWVLYELPNYRGRQYLLRPQEYRRCQDWGAMDAKAGSLRRVVDLY
Gene ID
Swiss Prot
SynonymsCCL; CRYG3; CTRCT2
Calculated MW21kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, U-251MG
Cellular locationcytoplasm, nucleus

Documents

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    ABclonal:Western blot - CRYGC Rabbit pAb (A9324)}

    Western blot - CRYGC Rabbit pAb (A9324)

    Western blot analysis of extracts of various cell lines, using CRYGC antibody (A9324) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - CRYGC Rabbit pAb (A9324)}

    Immunofluorescence - CRYGC Rabbit pAb (A9324)

    Immunofluorescence analysis of L929 cells using CRYGC antibody (A9324) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - CRYGC Rabbit pAb (A9324)}

    Immunofluorescence - CRYGC Rabbit pAb (A9324)

    Immunofluorescence analysis of C6 cells using CRYGC antibody (A9324) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.