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CSRP2BP Rabbit pAb (A7380)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - CSRP2BP Rabbit pAb (A7380)

Western blot analysis of various lysates, using CSRP2BP Rabbit pAb (A7380) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - CSRP2BP Rabbit pAb (A7380)

Immunofluorescence analysis of A549 cells using CSRP2BP antibody (A7380). Blue: DAPI for nuclear staining.

Overview

Product nameCSRP2BP Rabbit pAb
Catalog No.A7380
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CSRP2 is a protein containing two LIM domains, which are double zinc finger motifs found in proteins of diverse function. CSRP2 and some related proteins are thought to act as protein adapters, bridging two or more proteins to form a larger protein complex. The protein encoded by this gene binds to one of the LIM domains of CSRP2 and contains an acetyltransferase domain. Although the encoded protein has been detected in the cytoplasm, it is predominantly a nuclear protein. Alternatively spliced transcript variants have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 523-782 of human CSRP2BP (NP_065397.3).
SequenceDGIYGAKEGGISRLPAGQATYRTTCQDFRILDRYQTSLPSRKGFRHQTTKFLYRLVGSEDMAVDQSIVSPYTSRILKPYIRRDYETKPPKLQLLSQIRSHLHRSDPHWTPEPDAPLDYCYVRPNHIPTINSMCQEFFWPGIDLSECLQYPDFSVVVLYKKVIIAFGFMVPDVKYNEAYISFLFVHPEWRRAGIATFMIYHLIQTCMGKDVTLHVSASNPAMLLYQKFGFKTEEYVLDFYDKYYPLESTECKHAFFLRLRR
Gene ID
Swiss Prot
SynonymsATAC2; CRP2BP; CSRP2BP; PRO1194; dJ717M23.1
Calculated MW89kDa
Observed MW120kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat kidney
Cellular locationCytoplasm, Nucleus

Documents

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    ABclonal:Western blot - CSRP2BP Rabbit pAb (A7380)}

    Western blot - CSRP2BP Rabbit pAb (A7380)

    Western blot analysis of various lysates, using CSRP2BP Rabbit pAb (A7380) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - CSRP2BP Rabbit pAb (A7380)}

    Immunofluorescence - CSRP2BP Rabbit pAb (A7380)

    Immunofluorescence analysis of A549 cells using CSRP2BP antibody (A7380). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)