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CTIP2/BCL11B Rabbit mAb (A19804)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CTIP2/BCL11B Rabbit mAb (A19804)

Western blot analysis of various lysates using CTIP2/BCL11B Rabbit mAb (A19804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - CTIP2/BCL11B Rabbit mAb (A19804)

Immunohistochemistry analysis of paraffin-embedded mouse brain using CTIP2/BCL11B Rabbit mAb (A19804) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameCTIP2/BCL11B Rabbit mAb
Catalog No.A19804
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2329
This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-550 of human CTIP2/BCL11B (Q9C0K0).
SequenceTGEKPYKCQLCDHACSQASKLKRHMKTHMHKAGSLAGRSDDGLSAASSPEPGTSELAGEGLKAADGDFRHHESDPSLGHEPEEEDEEEEEEEEELLLENES
Gene ID
Swiss Prot
SynonymsATL1; RIT1; CTIP2; IMD49; CTIP-2; IDDFSTA; SMARCM2; ZNF856B; ATL1-beta; ATL1-alpha; ATL1-delta; ATL1-gamma; hRIT1-alpha; CTIP2/BCL11B
Calculated MW96kDa
Observed MW120-130kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, Raji, SH-SY5Y
Cellular locationNucleus
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - CTIP2/BCL11B Rabbit mAb (A19804)}

Western blot - CTIP2/BCL11B Rabbit mAb (A19804)

Western blot analysis of various lysates using CTIP2/BCL11B Rabbit mAb (A19804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - CTIP2/BCL11B Rabbit mAb (A19804)}

Immunohistochemistry - CTIP2/BCL11B Rabbit mAb (A19804)

Immunohistochemistry analysis of paraffin-embedded mouse brain using CTIP2/BCL11B Rabbit mAb (A19804) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)