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CTNNBIP1 Rabbit mAb (A0914)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CTNNBIP1 Rabbit mAb (A0914)

Western blot analysis of extracts of TE-1 cells, using CTNNBIP1 antibody (A0914) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameCTNNBIP1 Rabbit mAb
Catalog No.A0914
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2551
The protein encoded by this gene binds CTNNB1 and prevents interaction between CTNNB1 and TCF family members. The encoded protein is a negative regulator of the Wnt signaling pathway. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-81 of human CTNNBIP1 (Q9NSA3).
SequenceMNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSIDQGAEDVVMAFSRSETEDRRQ
Gene ID
Swiss Prot
SynonymsICAT
Calculated MW9kDa
Observed MW11kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesTE-1, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - CTNNBIP1 Rabbit mAb (A0914)}

Western blot - CTNNBIP1 Rabbit mAb (A0914)

Western blot analysis of extracts of TE-1 cells, using CTNNBIP1 antibody (A0914) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0914? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)