제품 > 항체 > 다중클론항체(pAb)

CTNNBL1 Rabbit pAb (A6572)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CTNNBL1 Rabbit pAb (A6572)

Western blot analysis of various lysates using CTNNBL1 Rabbit pAb (A6572) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CTNNBL1 Rabbit pAb (A6572)

Immunofluorescence analysis of U2OS cells using CTNNBL1 Rabbit pAb (A6572). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCTNNBL1 Rabbit pAb
Catalog No.A6572
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a component of the pre-mRNA-processing factor 19-cell division cycle 5-like (PRP19-CDC5L) protein complex, which activates pre-mRNA splicing and is an integral part of the spliceosome. The encoded protein is also a nuclear localization sequence binding protein, and binds to activation-induced deaminase and is important for antibody diversification. This gene may also be associated with the development of obesity. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 264-563 of human CTNNBL1 (NP_110517.2).
SequenceEVLAILLQDNDENRELLGELDGIDVLLQQLSVFKRHNPSTAEEQEMMENLFDSLCSCLMLSSNRERFLKGEGLQLMNLMLREKKISRSSALKVLDHAMIGPEGTDNCHKFVDILGLRTIFPLFMKSPRKIKKVGTTEKEHEEHVCSILASLLRNLRGQQRTRLLNKFTENDSEKVDRLMELHFKYLGAMQVADKKIEGEKHDMVRRGEIIDNDTEEEFYLRRLDAGLFVLQHICYIMAEICNANVPQIRQRVHQILNMRGSSIKIVRHIIKEYAENIGDGRSPEFRENEQKRILGLLENF
Gene ID
Swiss Prot
SynonymsNAP; P14L; IMD99; PP8304; C20orf33; dJ633O20.1; CTNNBL1
Calculated MW65kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, HeLa, SW620, HepG2, Mouse thymus, Rat brain
Cellular locationCytoplasm, Nucleus

Documents

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    ABclonal:Western blot - CTNNBL1 Rabbit pAb (A6572)}

    Western blot - CTNNBL1 Rabbit pAb (A6572)

    Western blot analysis of various lysates using CTNNBL1 Rabbit pAb (A6572) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - CTNNBL1 Rabbit pAb (A6572)}

    Immunofluorescence - CTNNBL1 Rabbit pAb (A6572)

    Immunofluorescence analysis of U2OS cells using CTNNBL1 Rabbit pAb (A6572). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.