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CTRL Rabbit mAb (A19660)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CTRL Rabbit mAb (A19660)

Western blot analysis of various lysates using CTRL Rabbit mAb (A19660) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - CTRL Rabbit mAb (A19660)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using CTRL Rabbit mAb (A19660) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CTRL Rabbit mAb (A19660)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using CTRL Rabbit mAb (A19660) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CTRL Rabbit mAb (A19660)

Confocal imaging of paraffin-embedded Human pancreas tissue using CTRL Rabbit mAb (A19660, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - CTRL Rabbit mAb (A19660)

Confocal imaging of paraffin-embedded Mouse pancreas tissue using CTRL Rabbit mAb (A19660, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - CTRL Rabbit mAb (A19660)

Confocal imaging of paraffin-embedded Rat pancreas tissue using CTRL Rabbit mAb (A19660, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameCTRL Rabbit mAb
Catalog No.A19660
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2218
This gene encodes a serine-type endopeptidase with chymotrypsin- and elastase-2-like activities. The gene encoding this zymogen is expressed specifically in the pancreas and likely functions as a digestive enzyme.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 165-264 of human CTRL (P40313).
SequenceGVGNVTPAHLQQVALPLVTVNQCRQYWGSSITDSMICAGGAGASSCQGDSGGPLVCQKGNTWVLIGIVSWGTKNCNVRAPAVYTRVSKFSTWINQVIAYN
Gene ID
Swiss Prot
SynonymsCTRL1; CTRL
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:8000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse pancreas, Rat pancreas
Cellular locationextracellular space

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CTRL Rabbit mAb (A19660)}

    Western blot - CTRL Rabbit mAb (A19660)

    Western blot analysis of various lysates using CTRL Rabbit mAb (A19660) at 1:5000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - CTRL Rabbit mAb (A19660)}

    Immunohistochemistry - CTRL Rabbit mAb (A19660)

    Immunohistochemistry analysis of paraffin-embedded mouse pancreas using CTRL Rabbit mAb (A19660) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - CTRL Rabbit mAb (A19660)}

    Immunohistochemistry - CTRL Rabbit mAb (A19660)

    Immunohistochemistry analysis of paraffin-embedded rat pancreas using CTRL Rabbit mAb (A19660) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - CTRL Rabbit mAb (A19660)}

    Immunofluorescence - CTRL Rabbit mAb (A19660)

    Confocal imaging of paraffin-embedded Human pancreas tissue using CTRL Rabbit mAb (A19660, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - CTRL Rabbit mAb (A19660)}

    Immunofluorescence - CTRL Rabbit mAb (A19660)

    Confocal imaging of paraffin-embedded Mouse pancreas tissue using CTRL Rabbit mAb (A19660, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - CTRL Rabbit mAb (A19660)}

    Immunofluorescence - CTRL Rabbit mAb (A19660)

    Confocal imaging of paraffin-embedded Rat pancreas tissue using CTRL Rabbit mAb (A19660, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)