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CYP4V2 Rabbit pAb (A6573)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CYP4V2 Rabbit pAb (A6573)

Western blot analysis of various lysates, using CYP4V2 Rabbit pAb (A6573) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - CYP4V2 Rabbit pAb (A6573)

Immunofluorescence analysis of NIH/3T3 cells using CYP4V2 Rabbit pAb (A6573) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CYP4V2 Rabbit pAb (A6573)

Immunofluorescence analysis of U-87MG cells using CYP4V2 Rabbit pAb (A6573) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCYP4V2 Rabbit pAb
Catalog No.A6573
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cytochrome P450 hemethiolate protein superfamily which are involved in oxidizing various substrates in the metabolic pathway. It is implicated in the metabolism of fatty acid precursors into n-3 polyunsaturated fatty acids. Mutations in this gene result in Bietti crystalline corneoretinal dystrophy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human CYP4V2 (NP_997235.3).
SequenceICETAMGKNIGAQSNDDSEYVRAVYRMSEMIFRRIKMPWLWLDLWYLMFKEGWEHKKSLQILHTFTNSVIAERANEMNANEDCRGDGRGSAPSKNKRRAFLDLLLSVTDDEGNRLSHEDIREEVDTFMFEGHDTTAAAINWSLYLLGSNPEVQKKVDHELDDVFGKSDRPATVEDLKKLRYLECVIKETLRLFPSVPLFARSVSEDCEVAGYRVLKGTEAVIIPYALHRDPRYFPNPEEFQPERFFPENAQGRHPYAYVPFSAGPRNCIGQKFAVMEEKTILSCILRHFWIESNQKREEL
Gene ID
Swiss Prot
SynonymsBCD; CYP4AH1; CYP4V2
Calculated MW61kDa
Observed MW61kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, U-87MG, Mouse liver, Rat liver
Cellular locationEndoplasmic reticulum membrane, Single-pass membrane protein.

Documents

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ABclonal:Western blot - CYP4V2 Rabbit pAb (A6573)}

Western blot - CYP4V2 Rabbit pAb (A6573)

Western blot analysis of various lysates, using CYP4V2 Rabbit pAb (A6573) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - CYP4V2 Rabbit pAb (A6573)}

Immunofluorescence - CYP4V2 Rabbit pAb (A6573)

Immunofluorescence analysis of NIH/3T3 cells using CYP4V2 Rabbit pAb (A6573) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CYP4V2 Rabbit pAb (A6573)}

Immunofluorescence - CYP4V2 Rabbit pAb (A6573)

Immunofluorescence analysis of U-87MG cells using CYP4V2 Rabbit pAb (A6573) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.