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Calmegin Rabbit mAb (A19643)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Calmegin Rabbit mAb (A19643)

Western blot analysis of various lysates, using Calmegin Rabbit mAb (A19643) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Calmegin Rabbit mAb (A19643)

Western blot analysis of extracts from Rat testis, using Calmegin Rabbit mAb (A19643) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Calmegin Rabbit mAb (A19643)

Immunofluorescence analysis of rat testis using Calmegin Rabbit mAb (A19643) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Calmegin Rabbit mAb (A19643)

Immunofluorescence analysis of mouse testis using Calmegin Rabbit mAb (A19643) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCalmegin Rabbit mAb
Catalog No.A19643
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2213
Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 180-350 of human Calmegin (O14967).
SequenceFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPA
Gene ID
Swiss Prot
Synonyms
Calculated MW70kDa
Observed MW93kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesPC-3, Mouse testis, Rat testis
Cellular locationendoplasmic reticulum

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Calmegin Rabbit mAb (A19643)}

    Western blot - Calmegin Rabbit mAb (A19643)

    Western blot analysis of various lysates, using Calmegin Rabbit mAb (A19643) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Calmegin Rabbit mAb (A19643)}

    Western blot - Calmegin Rabbit mAb (A19643)

    Western blot analysis of extracts from Rat testis, using Calmegin Rabbit mAb (A19643) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - Calmegin Rabbit mAb (A19643)}

    Immunofluorescence - Calmegin Rabbit mAb (A19643)

    Immunofluorescence analysis of rat testis using Calmegin Rabbit mAb (A19643) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Calmegin Rabbit mAb (A19643)}

    Immunofluorescence - Calmegin Rabbit mAb (A19643)

    Immunofluorescence analysis of mouse testis using Calmegin Rabbit mAb (A19643) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)