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Calnexin Rabbit mAb (A4846)

Publications (21) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Calnexin Rabbit mAb (A4846)

Western blot analysis of various lysates using Calnexin Rabbit mAb (A4846) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameCalnexin Rabbit mAb
Catalog No.A4846
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0648
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 493-592 of human Calnexin (P27824).
SequenceLPVFLVILFCCSGKKQTSGMEYKKTDAPQPDVKEEEEEKEEEKDKGDEEEEGEEKLEEKQKSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
Gene ID
Swiss Prot
SynonymsCNX; P90; IP90; Calnexin
Calculated MW68kDa
Observed MW90kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HepG2, K-562
Cellular locationEndoplasmic reticulum, Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus, Mus musculus, Sus scrofa, Other)

Documents

Certificate of Compliance

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ABclonal:Western blot - Calnexin Rabbit mAb (A4846)}

Western blot - Calnexin Rabbit mAb (A4846)

Western blot analysis of various lysates using Calnexin Rabbit mAb (A4846) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4846? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

ELISA 키트 (1)

Secondary Antibodies (26)