제품 > 항체 > 다중클론항체(pAb)

Calnexin Rabbit pAb (A15631)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Calnexin Rabbit pAb (A15631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Calnexin Rabbit pAb (A15631)

Immunofluorescence analysis of PC-12 cells using Calnexin Rabbit pAb (A15631) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Calnexin Rabbit pAb
Catalog No.	A15631
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 500-592 of human Calnexin (NP_001737.1).
Sequence	LFCCSGKKQTSGMEYKKTDAPQPDVKEEEEEKEEEKDKGDEEEEGEEKLEEKQKSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
Gene ID
Swiss Prot
Synonyms	CNX; P90; IP90; Calnexin
Calculated MW	68kDa
Observed MW	90kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	MCF7, HeLa, HepG2, Jurkat, Mouse brain, Mouse liver, Rat lung, Rat brain
Cellular location	Endoplasmic reticulum, Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein
Customer validation	WB(Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus, Mus musculus) IF(Homo sapiens, Mus musculus) IP(Homo sapiens) IF(Homo sapiens) WB(Mus musculus)

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Research Area

연구후성유전학 & 핵 신호전달RNA 결합

Calnexin Rabbit pAb images

Western blot - Calnexin Rabbit pAb (A15631)

Immunofluorescence - Calnexin Rabbit pAb (A15631)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Theranostics

Application:

IF:

12.4

Species:

Homo sapiens

PMID:

31149048

Title:

Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction.

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Molecules

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

32481657

Title:

A Comparative Quantitative Proteomic Analysis of HCMV-Infected Cells Highlights pUL138 as a Multifunctional Protein

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Reprod Domest Anim

Application:

IF:

1.6

Species:

Gallus gallus

PMID:

34717018

Title:

Characterization and proteomics of chicken seminal plasma extracellular vesicles

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Ann Transl Med

Application:

IF:

Species:

Homo sapiens

PMID:

34988181

Title:

Phenotype and molecular characterizations of a family with dentinogenesis imperfecta shields type II with a novel DSPP mutation

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

35761332

Title:

Delivery of engineered extracellular vesicles with miR-29b editing system for muscle atrophy therapy - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Journal of inflammation (London, England)

Application:

IF:

4.4

Species:

Homo sapiens

PMID:

36324164

Title:

Extracellular vesicles isolated from hyperuricemia patients might aggravate airway inflammation of COPD via senescence-associated pathway - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

Species:

Mus musculus

PMID:

36307906

Title:

Cell‐Taxi: Mesenchymal Cells Carry and Transport Clusters of Cancer Cells

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Genes

Application:

IF:

3.5

Species:

Mus musculus

PMID:

36672913

Title:

Functional Characterization of a Phf8 Processed Pseudogene in the Mouse Genome - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Cancer cell international

Application:

IF:

6.4

Species:

Homo sapiens

PMID:

37158903

Title:

Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

37506505

Title:

HSF1 enhances the attenuation of exosomes from mesenchymal stem cells to hemorrhagic shock induced lung injury by altering the protein profile of

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Research square

Application:

IF:

Species:

Homo sapiens

PMID:

37886538

Title:

LDHA-regulated tumor-macrophage symbiosis promotes glioblastoma progression - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

2.5

Species:

Rattus norvegicus

PMID:

37826943

Title:

Exosomes derived from cardiac fibroblasts with angiotensin II stimulation provoke hypertrophy and autophagy inhibition in cardiomyocytes

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Cells

Application:

IF:

7.7

Species:

Homo sapiens, Mus musculus

PMID:

34207793

Title:

iPLA2 Contributes to ER Stress-Induced Apoptosis during Myocardial Ischemia/Reperfusion Injury

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Frontiers in bioengineering and biotechnology

Application:

IF:

4.3

Species:

Homo sapiens, Mus musculus

PMID:

35615474

Title:

A Potential Target for Diabetic Vascular Damage: High Glucose-Induced Monocyte Extracellular Vesicles Impair Endothelial Cells by Delivering miR-142-5p

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Gut

Application:

IF:

24.5

Species:

Homo sapiens

PMID:

37973365

Title:

Chimeric antigen receptors of HBV envelope proteins inhibit hepatitis B surface antigen secretion

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Cell death and differentiation

Application:

IF:

13.7

Species:

Homo sapiens

PMID:

35962186

Title:

Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Cell reports. Medicine

Application:

WB, IF

IF:

11.7

Species:

Homo sapiens

PMID:

37075705

Title:

Deficiency of the CD155-CD96 immune checkpoint controls IL-9 production in giant cell arteritis - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Circulation Research

Application:

IF:

20.1

Species:

Homo sapiens

PMID:

37589160

Title:

Endothelial FIS1 DeSUMOylation Protects Against Hypoxic Pulmonary Hypertension

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Biomedicines

Application:

IF:

4.7

Species:

Mus musculus

PMID:

38255205

Title:

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Rescue Testicular Aging - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

ACS nano

Application:

IF:

15.8

Species:

Rattus norvegicus

PMID:

38290527

Title:

A Dual Role of Mesenchymal Stem Cell Derived Small Extracellular Vesicles on TRPC6 Protein and Mitochondria to Promote Diabetic Wound Healing - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Mus musculus

PMID:

38443336

Title:

Lactate dehydrogenase A regulates tumor-macrophage symbiosis to promote glioblastoma progression - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

mBio

Application:

IF:

5.1

Species:

Mus musculus

PMID:

38349129

Title:

Lyssavirus M protein degrades neuronal microtubules by reprogramming mitochondrial metabolism

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Stem cell research & therapy

Application:

IF:

8.1

Species:

Homo sapiens

PMID:

38566259

Title:

Manufacturing, quality control, and GLP-grade preclinical study of nebulized allogenic adipose mesenchymal stromal cells-derived extracellular vesicles

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Cell Cycle

Application:

IF:

3.4

Species:

Rattus norvegicus

PMID:

33685347

Title:

Exosomes derived from autologous dermal fibroblasts promote diabetic cutaneous wound healing through the Akt/-catenin pathway

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Stem cell research & therapy

Application:

IF:

8.1

Species:

Rattus norvegicus

PMID:

35841008

Title:

BMSC-derived exosomes promote tendon-bone healing after anterior cruciate ligament reconstruction by regulating M1/M2 macrophage polarization in rats - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Molecular cell

Application:

IF:

14.5

Species:

Homo sapiens,Mus musculus

PMID:

37751742

Title:

Lysosomal cyst (e) ine storage potentiates tolerance to oxidative stress in cancer cells

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Bioactive materials

Application:

IF:

18.9

Species:

Mus musculus

PMID:

39247401

Title:

Bioprinted mesenchymal stem cell microfiber-derived extracellular vesicles alleviate unilateral renal ischemia-reperfusion injury and fibrosis by inhibiting tubular epithelial cells ferroptosis - PMC

References for Calnexin Rabbit pAb (A15631)

Product:

Calnexin Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

39407245