제품 > 항체 > 단일클론항체(mAb)

Calsequestrin 1 Rabbit mAb (A19640)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of extracts of Rat heart, using Calsequestrin 1 Rabbit mAb (A19640) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of Mouse skeletal muscle, using Calsequestrin 1 Rabbit mAb (A19640) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Calsequestrin 1 Rabbit mAb (A19640)

Immunofluorescence analysis of Mouse skeletal muscle using Calsequestrin 1 antibody (A19640) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Rat skeletal muscle using Calsequestrin 1 antibody (A19640) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	Calsequestrin 1 Rabbit mAb
Catalog No.	A19640
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2209

Background

This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_001222.3).
Sequence	EDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDA
Gene ID
Swiss Prot
Synonyms	CASQ; CSQ1; PDIB1; VMCQA
Calculated MW	45kDa
Observed MW	55kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse skeletal muscle, Rat heart
Cellular location	Endoplasmic reticulum, Mitochondrial matrix
Customer validation	WB(Homo sapiens)