제품 > 항체 > 단일클론항체(mAb)

Carbonic Anhydrase 1 (CA1) Rabbit mAb (A4406)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Carbonic Anhydrase 1 (CA1) Rabbit mAb (A4406)

Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (CA1) (CA1) Rabbit mAb (A4406) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameCarbonic Anhydrase 1 (CA1) Rabbit mAb
Catalog No.A4406
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1062
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-261 of human Carbonic Anhydrase 1 (CA1) (NP_001729.1).
SequenceKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF
Gene ID
Swiss Prot
SynonymsCAB; CA-I; Car1; HEL-S-11
Calculated MW29kDa
Observed MW29kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Mouse spleen, Rat liver, Rat kidney
Cellular locationCytoplasm
Customer validation

WB(Scylla)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Carbonic Anhydrase 1 (CA1) Rabbit mAb (A4406)}

Western blot - Carbonic Anhydrase 1 (CA1) Rabbit mAb (A4406)

Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (CA1) (CA1) Rabbit mAb (A4406) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4406? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)