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Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Western blot analysis of various lysates using Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameCarbonic Anhydrase 1 (CA1) Rabbit pAb
Catalog No.A1031
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) (NP_001122303.1).
SequenceMASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF
Gene ID
Swiss Prot
SynonymsCAB; CA-I; Car1; HEL-S-11; Carbonic Anhydrase 1 (CA1)
Calculated MW29kDa
Observed MW30kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Mouse liver, Mouse heart, Mouse brain
Cellular locationCytoplasm.
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)}

Western blot - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Western blot analysis of various lysates using Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)