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Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934)

Review (2)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934)

Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb(A4934)
Review
ABclonal: review for Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb(A4934)

Overview

Product nameCarbonic Anhydrase 9 (CA9/G250) Rabbit mAb
Catalog No.A4934
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1275
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human Carbonic Anhydrase 9 (CA9/G250) (Q16790).
SequenceHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIA
Gene ID
Swiss Prot
SynonymsMN; CAIX; Carbonic Anhydrase 9 (CA9/G250)
Calculated MW50kDa
Observed MW35-58kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHT-29, SW620
Cellular locationCell membrane, Cell projection, Nucleus, Single-pass type I membrane protein, microvillus membrane, nucleolus
Customer validation

(PC-3 、HepG2, PC-3 HepG2)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934)}

Western blot - Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934)

Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934)}

Immunohistochemistry - Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (A4934) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

재조합 단백질 (1)

Secondary Antibodies (26)