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Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of various lysates using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Western blot analysis of lysates from SGC-7901 cells using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb
Catalog No.	A1014
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-391 of human Casein Kinase 2 alpha (CSNK2A1) (NP_001886.1).
Sequence	MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ
Gene ID
Swiss Prot
Synonyms	CKII; Cka1; Cka2; CK2A1; OCNDS; Casein Kinase 2 alpha (CSNK2A1)
Calculated MW	45kDa
Observed MW	41kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	SGC-7901, H460, HT-1080, BT-474, SW620, Mouse liver, Mouse heart, Rat liver, Mouse heart, Rat liver, SGC-7901
Cellular location	Nucleus.
Customer validation	IHC(Homo sapiens, Mus musculus) IF(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb images

Western blot - Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014)

Western blot analysis of various lysates using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Western blot - Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014)

Immunohistochemistry - Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014)

* For research use only. Not for therapeutic or diagnostic purposes.