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Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (AP0335)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (AP0335)

Western blot analysis of lysates from 293F cells using Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (AP0335) at 1:400 dilution. 293F cells were treated by ERK2 (100ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product namePhospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb
Catalog No.AP0335
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding T360/S362 of human Casein Kinase 2 alpha (CSNK2A1)
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsCKII; Cka1; Cka2; CK2A1; OCNDS; Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F treated by ERK2
Cellular locationNucleus.
Customer validation

WB(Mus musculus)

IHC(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (AP0335)}

Western blot - Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (AP0335)

Western blot analysis of lysates from 293F cells using Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (AP0335) at 1:400 dilution. 293F cells were treated by ERK2 (100ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)