제품 > 항체 > 다중클론항체(pAb)

Cathepsin B Rabbit pAb (A0967)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Cathepsin B Rabbit pAb (A0967) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from Mouse thymus, using Cathepsin B Rabbit pAb (A0967) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Cathepsin B Rabbit pAb (A0967)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Cathepsin B Rabbit pAb (A0967) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Cathepsin B Rabbit pAb (A0967) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Cathepsin B Rabbit pAb (A0967) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Cathepsin B Rabbit pAb (A0967)

Immunofluorescence analysis of NIH/3T3 cells using Cathepsin B Rabbit pAb (A0967) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Cathepsin B Rabbit pAb (A0967) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Cathepsin B Rabbit pAb
Catalog No.	A0967
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Both Cathepsin B and Cathepsin L are involved in the cleavage of the spike protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upon its entry to the human host cell. Multiple pseudogenes of this gene have been identified.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-339 of human Cathepsin B (NP_001899.1).
Sequence	RSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI
Gene ID
Swiss Prot
Synonyms	KWE; APPS; CPSB; RECEUP; Cathepsin B
Calculated MW	38kDa
Observed MW	24kDa/27kDa/44kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2, A375, HT-1080, Rat kidney, Mouse thymus
Cellular location	Lysosome, Melanosome, Secreted, extracellular space.
Customer validation	WB(Homo sapiens, Mus musculus, Sus scrofa, Rattus norvegicus) IF(Homo sapiens) FC(Rattus norvegicus)

Documents

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Research Area

Cathepsin B Rabbit pAb images

Western blot - Cathepsin B Rabbit pAb (A0967)

Immunohistochemistry - Cathepsin B Rabbit pAb (A0967)

Immunofluorescence - Cathepsin B Rabbit pAb (A0967)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

J Nat Med

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

33188510

Title:

Isoorientin exerts a urate-lowering effect through inhibition of xanthine oxidase and regulation of the TLR4-NLRP3 inflammasome signaling pathway

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

Brain plasticity (Amsterdam, Netherlands)

Application:

IF:

Species:

Homo sapiens

PMID:

36448040

Title:

High Intensity Acute Aerobic Exercise Elicits Alterations in Circulating and Skeletal Muscle Tissue Expression of Neuroprotective Exerkines - PMC

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

37201357

Title:

Maintenance of cathepsin D-dependent autophagy-lysosomal function protects against cardiac ischemia/reperfusion injury

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

Autophagy

Application:

IF:

14.6

Species:

Mus musculus

PMID:

37584546

Title:

The presence of blastocyst within the uteri facilitates lumenal epithelium transformation for implantation via upregulating lysosome proteostasis activity

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

BioFactors (Oxford, England)

Application:

IF:

Species:

Sus scrofa

PMID:

35286732

Title:

Autophagy flux inhibition mediated by lysosomal dysfunction participates in the cadmium exposure-induced cardiotoxicity in swine

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

Archives of toxicology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

36826473

Title:

Lysosomal dysfunction in Schwann cells is involved in bortezomib-induced peripheral neuropathy

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

Toxicology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38710382

Title:

ROS-mediated NRF2/p-ERK1/2 signaling-involved mitophagy contributes to macrophages activation induced by CdTe quantum dots

References for Cathepsin B Rabbit pAb (A0967)

Product:

Cathepsin B Rabbit pAb

Journal:

Journal of hazardous materials

Application:

IF:

12.2

Species:

Rattus norvegicus

PMID:

38335616

Title:

The role of mTORC1/TFEB axis mediated lysosomal biogenesis and autophagy impairment in fluoride neurotoxicity and the intervention effects of resveratrol