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Cathepsin E (CTSE) Rabbit pAb (A2678)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cathepsin E (CTSE) Rabbit pAb (A2678)

Western blot analysis of various lysates using Cathepsin E (CTSE) Rabbit pAb (A2678) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Cathepsin E (CTSE) Rabbit pAb (A2678) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)

Immunohistochemistry analysis of paraffin-embedded Human lung using Cathepsin E (CTSE) Rabbit pAb (A2678) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Cathepsin E (CTSE) Rabbit pAb (A2678) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameCathepsin E (CTSE) Rabbit pAb
Catalog No.A2678
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 132-244 of human Cathepsin E (CTSE) (P14091).
SequenceGQSFSIQYGTGSLSGIIGADQVSVEGLTVVGQQFGESVTEPGQTFVDAEFDGILGLGYPSLAVGGVTPVFDNMMAQNLVDLPMFSVYMSSNPEGGAGSELIFGGYDHSHFSGS
Gene ID
Swiss Prot
SynonymsCATE; Cathepsin E (CTSE)
Calculated MW43kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse spleen, Mouse small intestine, Rat thymus
Cellular locationEndosome.

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    ABclonal:Western blot - Cathepsin E (CTSE) Rabbit pAb (A2678)}

    Western blot - Cathepsin E (CTSE) Rabbit pAb (A2678)

    Western blot analysis of various lysates using Cathepsin E (CTSE) Rabbit pAb (A2678) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)}

    Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using Cathepsin E (CTSE) Rabbit pAb (A2678) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)}

    Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)

    Immunohistochemistry analysis of paraffin-embedded Human lung using Cathepsin E (CTSE) Rabbit pAb (A2678) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)}

    Immunohistochemistry - Cathepsin E (CTSE) Rabbit pAb (A2678)

    Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Cathepsin E (CTSE) Rabbit pAb (A2678) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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