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Cenexin1 / ODF2 Rabbit mAb (A3607)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Cenexin1 / ODF2 Rabbit mAb (A3607)

Western blot analysis of extracts of various cell lines, using Cenexin1 / ODF2 antibody (A3607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameCenexin1 / ODF2 Rabbit mAb
Catalog No.A3607
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2057
The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 730-829 of human Cenexin1 / ODF2 (Q5BJF6).
SequenceKEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRLQDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPPA
Gene ID
Swiss Prot
SynonymsCT134; ODF84; ODF2/1; ODF2/2
Calculated MW95kDa
Observed MW97kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HepG2, PC-3
Cellular locationcentriolar subdistal appendage, centriole, centrosome, ciliary basal body, ciliary transition fiber, cilium, cytosol, nucleus, sperm flagellum, spindle pole

Documents

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ABclonal:Western blot - Cenexin1 / ODF2 Rabbit mAb (A3607)}

Western blot - Cenexin1 / ODF2 Rabbit mAb (A3607)

Western blot analysis of extracts of various cell lines, using Cenexin1 / ODF2 antibody (A3607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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