제품 > 항체 > 다중클론항체(pAb)

Ch25h Rabbit pAb (A24299)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from Mouse lung, using Ch25h Rabbit pAb (A24299) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	Ch25h Rabbit pAb
Catalog No.	A24299
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables cholesterol 25-hydroxylase activity. Involved in B cell chemotaxis. Acts upstream of or within cholesterol metabolic process. Predicted to be located in endoplasmic reticulum. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum membrane. Is expressed in several structures, including genitourinary system; gut; hemolymphoid system gland; respiratory system; and skin. Orthologous to human CH25H (cholesterol 25-hydroxylase).

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 171-270 of human Ch25h (NP_003947.1).
Sequence	TQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVP
Gene ID
Swiss Prot
Synonyms	m25OH; Ch25h
Calculated MW	31kDa
Observed MW	32kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse lung
Cellular location	Endoplasmic reticulum membrane, Multi-pass membrane protein