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Claudin-3 Rabbit mAb (A24603)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Claudin-3 Rabbit mAb (A24603)

Western blot analysis of various lysates, using Claudin-3 Rabbit mAb (A24603) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human oophoroma tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Claudin-3 Rabbit mAb (A24603)

Confocal imaging of MCF7 cells using Claudin-3 Rabbit mAb (A24603, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

ABclonal:Immunofluorescence - Claudin-3 Rabbit mAb (A24603)

Confocal imaging of T-47D cells using Claudin-3 Rabbit mAb (A24603, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameClaudin-3 Rabbit mAb
Catalog No.A24603
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64060
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 121-220 of human Claudin-3 mAb (NP_001297.1).
SequenceGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV
Gene ID
Swiss Prot
SynonymsRVP1; HRVP1; C7orf1; CPE-R2; CPETR2; Claudin-3
Calculated MW23kDa
Observed MW20kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:800
  • IF 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29
Cellular locationCell junction, tight junction, cell membrane.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Claudin-3 Rabbit mAb (A24603)}

Western blot - Claudin-3 Rabbit mAb (A24603)

Western blot analysis of various lysates, using Claudin-3 Rabbit mAb (A24603) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)}

Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)}

Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)}

Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human oophoroma tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)}

Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)}

Immunohistochemistry - Claudin-3 Rabbit mAb (A24603)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Claudin-3 Rabbit mAb (A24603) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Claudin-3 Rabbit mAb (A24603)}

Immunofluorescence - Claudin-3 Rabbit mAb (A24603)

Confocal imaging of MCF7 cells using Claudin-3 Rabbit mAb (A24603, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ABclonal:Immunofluorescence - Claudin-3 Rabbit mAb (A24603)}

Immunofluorescence - Claudin-3 Rabbit mAb (A24603)

Confocal imaging of T-47D cells using Claudin-3 Rabbit mAb (A24603, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

ELISA 키트 (1)

Secondary Antibodies (26)