제품 > 항체 > 단일클론항체(mAb)

Cleaved IL-18 (Tyr37) Rabbit mAb (A24057)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from GSDMD knockout (KO) HeLa cells and GSDMD knockout (KO) HeLa cells transfected with LPS using Cleaved IL-18 (Tyr37) Rabbit mAb (A24057) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:60s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Western blot analysis of Recombinant Human IL-18 Protein (RP00006) using Cleaved IL-18 (Tyr37) Rabbit mAb (A24057) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 1 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Review

Overview

Product name	Cleaved IL-18 (Tyr37) Rabbit mAb
Catalog No.	A24057
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC64645

Background

The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to residues surrounding Tyr37 of human IL18 (NP_001553.1).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	mature IL-18; Cleaved IL-18 (Tyr37)
Calculated MW	22KDa
Observed MW	18KDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	GSDMD knockout (KO) HeLa cells transfected with LPS, Recombinant Human IL-18 Protein
Cellular location	Secreted, Cytoplasm.
Customer validation	(HeLa cells)