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Phospho-STK11-S334 Rabbit pAb (AP0601)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-STK11-S334 Rabbit pAb (AP0601) at 1:1000 dilution. Hela cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37℃ for 30 minutes after serum-starvation
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-STK11-S334 Rabbit pAb
Catalog No.	AP0601
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S334 of human STK11 (NP_000446.1).
Sequence	WRSMT
Gene ID
Swiss Prot
Synonyms	PJS; LKB1; hLKB1; Phospho-STK11-S334
Calculated MW	49kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by PMA, HeLa treated by UV and PMA
Cellular location	Cytoplasm, Membrane, Mitochondrion, Nucleus