제품 > 항체 > 단일클론항체(mAb)

Coagulation Protein C Rabbit mAb (A4532)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Coagulation Protein C Rabbit mAb (A4532)

Western blot analysis of various lysates using Coagulation Coagulation Protein C Rabbit mAb (A4532) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - Coagulation Protein C Rabbit mAb (A4532)

Confocal imaging of paraffin-embedded Mouse liver tissue using Coagulation Protein C Rabbit mAb (A4532, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameCoagulation Protein C Rabbit mAb
Catalog No.A4532
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1023
This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-461 of human Coagulation Coagulation Protein C (P04070).
SequenceIALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP
Gene ID
Swiss Prot
SynonymsPC; APC; PROC1; THPH3; THPH4; Coagulation Protein C
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHT-29, Mouse liver
Cellular locationEndoplasmic reticulum, Golgi apparatus, Secreted

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ABclonal:Western blot - Coagulation Protein C Rabbit mAb (A4532)}

Western blot - Coagulation Protein C Rabbit mAb (A4532)

Western blot analysis of various lysates using Coagulation Coagulation Protein C Rabbit mAb (A4532) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunofluorescence - Coagulation Protein C Rabbit mAb (A4532)}

Immunofluorescence - Coagulation Protein C Rabbit mAb (A4532)

Confocal imaging of paraffin-embedded Mouse liver tissue using Coagulation Protein C Rabbit mAb (A4532, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)