제품 > 항체 > 단일클론항체(mAb)

Collagen I/COL1A2 Rabbit mAb (A21059)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from Mouse bone, using Collagen I/COL1A2 Rabbit mAb (A21059) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from Rat skin, using Collagen I/COL1A2 Rabbit mAb (A21059) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - Collagen I/COL1A2 Rabbit mAb (A21059)

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A2 Rabbit mAb (A21059) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Collagen I/COL1A2 Rabbit mAb
Catalog No.	A21059
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC52128

Background

This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2 (NP_000080.2).
Sequence	PTGDPGKNGDKGHAGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLHGEFGLPGPAGPRGERGPPGESGAA
Gene ID
Swiss Prot
Synonyms	OI4; EDSCV; EDSARTH2; Collagen I/COL1A2
Calculated MW	129kDa
Observed MW	129kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse bone, Rat skin
Cellular location	Secreted, extracellular matrix, Extracellular space.
Customer validation	IF(Gallus gallus, Homo sapiens, Mus musculus) WB(Oryctolagus cuniculus, Mus musculus, Homo sapiens, Rattus norvegicus) WB(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

Collagen I/COL1A2 Rabbit mAb images

Western blot - Collagen I/COL1A2 Rabbit mAb (A21059)

Immunofluorescence - Collagen I/COL1A2 Rabbit mAb (A21059)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Gallus gallus

PMID:

36423455

Title:

Thiram-induced hyperglycemia causes tibial dyschondroplasia by triggering aberrant ECM remodeling via the gut-pancreas axis in broiler chickens

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Regenerative therapy

Application:

IF:

3.65

Species:

Oryctolagus cuniculus

PMID:

37292187

Title:

Bone marrow mesenchymal stem cell transplantation alleviates radiation-induced myocardial fibrosis through inhibition of the TGF-β1/Smad2/3 signaling pathway in rabbit model - PMC

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Biomedical reports

Application:

IF:

Species:

Mus musculus

PMID:

37954636

Title:

Octanoyl esterification of low molecular weight sulfated galactan enhances the cellular uptake and collagen expression in fibroblast cells - PMC

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Homo sapiens, Mus musculus

PMID:

37269686

Title:

EETs alleviate alveolar epithelial cell senescence by inhibiting endoplasmic reticulum stress through the Trim 25/Keap1/Nrf2 axis

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Cell transplantation

Application:

IF:

4.13

Species:

Homo sapiens

PMID:

37798942

Title:

1,25-Dihydroxyvitamin D3: A Positive Factor for the Osteogenic Differentiation of hPDLSCs and for the Tissue Regenerative Activity of Cell Sheets - PMC

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Mus musculus

PMID:

37416635

Title:

Mitochondrial citrate accumulation triggers senescence of alveolar epithelial cells contributing to pulmonary fibrosis in mice - PMC

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

6.2

Species:

Rattus norvegicus

PMID:

38810917

Title:

tRNA-derived small RNA (tsr007330) regulates myocardial fibrosis after myocardial infarction through NAT10-mediated ac4C acetylation of EGR3 mRNA

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

FEBS open bio

Application:

IF:

2.6

Species:

Mus musculus

PMID:

38710658

Title:

Regulation of cardiac fibrosis in mice with TAC/DOCAinduced HFpEF by resistinlike molecule gamma and adenylate cyclase 1

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Rattus norvegicus

PMID:

39143545

Title:

A structured biomimetic nanoparticle as inflammatory factor sponge and autophagy-regulatory agent against intervertebral disc degeneration and discogenic pain - PMC

References for Collagen I/COL1A2 Rabbit mAb (A21059)

Product:

Collagen I/COL1A2 Rabbit mAb

Journal:

Experimental eye research

Application:

IF, WB

IF:

Species:

Homo sapiens

PMID:

38272381