제품 > 항체 > 단일클론항체(mAb)

Cortactin Rabbit mAb (A9518)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cortactin Rabbit mAb (A9518)

Western blot analysis of various lysates using Cortactin Rabbit mAb (A9518) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - Cortactin Rabbit mAb (A9518)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Cortactin Rabbit mAb (A9518) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Cortactin Rabbit mAb (A9518)

Confocal imaging of paraffin-embedded Mouse colon tissue using Cortactin Rabbit mAb (A9518, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Cortactin Rabbit mAb (A9518)

Confocal imaging of paraffin-embedded Human stomach tissue using Cortactin Rabbit mAb (A9518, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunoprecipitation - Cortactin Rabbit mAb (A9518)

Immunoprecipitation analysis of 600 μg extracts of Mouse lung cells using 3 μg Cortactin antibody (A9518). Western blot was performed from the immunoprecipitate using Cortactin antibody (A9518) at a dilution of 1:1000.

Overview

Product nameCortactin Rabbit mAb
Catalog No.A9518
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1613
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cortactin (Q14247).
SequenceMWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAV
Gene ID
Swiss Prot
SynonymsEMS1; Cortactin
Calculated MW62kDa
Observed MW80-85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, HUVEC, Mouse lung
Cellular locationCell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein, cell cortex, clathrin-coated pit, cytoskeleton, dendrite, dendritic spine, focal adhesion, lamellipodium, podosome, ruffle.
Customer validation

IHC(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Cortactin Rabbit mAb (A9518)}

Western blot - Cortactin Rabbit mAb (A9518)

Western blot analysis of various lysates using Cortactin Rabbit mAb (A9518) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - Cortactin Rabbit mAb (A9518)}

Immunohistochemistry - Cortactin Rabbit mAb (A9518)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Cortactin Rabbit mAb (A9518) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Cortactin Rabbit mAb (A9518)}

Immunofluorescence - Cortactin Rabbit mAb (A9518)

Confocal imaging of paraffin-embedded Mouse colon tissue using Cortactin Rabbit mAb (A9518, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Cortactin Rabbit mAb (A9518)}

Immunofluorescence - Cortactin Rabbit mAb (A9518)

Confocal imaging of paraffin-embedded Human stomach tissue using Cortactin Rabbit mAb (A9518, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunoprecipitation - Cortactin Rabbit mAb (A9518)}

Immunoprecipitation - Cortactin Rabbit mAb (A9518)

Immunoprecipitation analysis of 600 μg extracts of Mouse lung cells using 3 μg Cortactin antibody (A9518). Western blot was performed from the immunoprecipitate using Cortactin antibody (A9518) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A9518? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)