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Cyclin E2 Rabbit pAb (A7032)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Cyclin E2 Rabbit pAb (A7032)

Western blot analysis of extracts of Jurkat cells, using Cyclin E2 antibody (A7032).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - Cyclin E2 Rabbit pAb (A7032)

Immunofluorescence analysis of MCF7 cells using Cyclin E2 antibody (A7032).

Overview

Product nameCyclin E2 Rabbit pAb
Catalog No.A7032
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 255-404 of human Cyclin E2 (NP_477097.1).
SequenceALKDAPKVLLPQYSQETFIQIAQLLDLCILAIDSLEFQYRILTAAALCHFTSIEVVKKASGLEWDSISECVDWMVPFVNVVKSTSPVKLKTFKKIPMEDRHNIQTHTNYLAMLEEVNYINTFRKGGQLSPVCNGGIMTPPKSTEKPPGKH
Gene ID
Swiss Prot
SynonymsCYCE2
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Cyclin E2 Rabbit pAb (A7032)}

Western blot - Cyclin E2 Rabbit pAb (A7032)

Western blot analysis of extracts of Jurkat cells, using Cyclin E2 antibody (A7032).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Cyclin E2 Rabbit pAb (A7032)}

Immunofluorescence - Cyclin E2 Rabbit pAb (A7032)

Immunofluorescence analysis of MCF7 cells using Cyclin E2 antibody (A7032).

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7032? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)