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Cyclin H Rabbit mAb (A4076)

Review (1)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Cyclin H Rabbit mAb (A4076)

Western blot analysis of various lysates using Cyclin H Rabbit mAb (A4076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Cyclin H Rabbit mAb (A4076)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Cyclin H Rabbit mAb (A4076) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Cyclin H Rabbit mAb (A4076)

Immunofluorescence analysis of NIH-3T3 cells using Cyclin H Rabbit mAb (A4076) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cyclin H Rabbit mAb (A4076)

Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit mAb (A4076) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Cyclin H Rabbit mAb(A4076)

Overview

Product nameCyclin H Rabbit mAb
Catalog No.A4076
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0893
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 224-323 of human Cyclin H (P51946).
SequenceAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL
Gene ID
Swiss Prot
SynonymsCAK; p34; p37; CycH; Cyclin H
Calculated MW38kDa
Observed MW34kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Hep G2, Jurkat, Mouse testis
Cellular locationNucleus
Customer validation

(HT29)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Cyclin H Rabbit mAb (A4076)}

Western blot - Cyclin H Rabbit mAb (A4076)

Western blot analysis of various lysates using Cyclin H Rabbit mAb (A4076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Cyclin H Rabbit mAb (A4076)}

Immunohistochemistry - Cyclin H Rabbit mAb (A4076)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Cyclin H Rabbit mAb (A4076) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Cyclin H Rabbit mAb (A4076)}

Immunofluorescence - Cyclin H Rabbit mAb (A4076)

Immunofluorescence analysis of NIH-3T3 cells using Cyclin H Rabbit mAb (A4076) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cyclin H Rabbit mAb (A4076)}

Immunofluorescence - Cyclin H Rabbit mAb (A4076)

Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit mAb (A4076) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)