제품 > 항체 > 다중클론항체(pAb)

Cyclin Y Rabbit pAb (A17845)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cyclin Y Rabbit pAb (A17845)

Western blot analysis of various lysates using Cyclin Y Rabbit pAb (A17845) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameCyclin Y Rabbit pAb
Catalog No.A17845
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cyclins, such as CCNY, control cell division cycles and regulate cyclin-dependent kinases (e.g., CDC2; MIM 116940) (Li et al., 2009 [PubMed 18060517]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-120 of human CCNY (NP_859049.2).
SequencePNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRF
Gene ID
Swiss Prot
SynonymsCCNX; CFP1; CBCP1; C10orf9; Cyclin Y
Calculated MW39kDa
Observed MW39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, C6
Cellular locationcytoplasmic cyclin-dependent protein kinase holoenzyme complex, nucleus, plasma membrane
ABclonal:Western blot - Cyclin Y Rabbit pAb (A17845)}

Western blot - Cyclin Y Rabbit pAb (A17845)

Western blot analysis of various lysates using Cyclin Y Rabbit pAb (A17845) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.