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Cytokeratin 9 (KRT9) Rabbit pAb (A10119)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)

Western blot analysis of lysates from Mouse brain, using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)

Immunofluorescence analysis of paraffin-embedded human skin using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)

Immunofluorescence analysis of paraffin-embedded mouse skin using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameCytokeratin 9 (KRT9) Rabbit pAb
Catalog No.A10119
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 150-450 of human Cytokeratin 9 (KRT9) (KRT9) (NP_000217.2).
SequenceTANEKSTMQELNSRLASYLDKVQALEEANNDLENKIQDWYDKKGPAAIQKNYSPYYNTIDDLKDQIVDLTVGNNKTLLDIDNTRMTLDDFRIKFEMEQNLRQGVDADINGLRQVLDNLTMEKSDLEMQYETLQEELMALKKNHKEEMSQLTGQNSGDVNVEINVAPGKDLTKTLNDMRQEYEQLIAKNRKDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLRHGVQELEIELQSQLSKKAALEKSLEDTKNRYCGQLQMIQEQISNLEAQITDVRQEIECQNQEYSLLLSIKMRLEKE
Gene ID
Swiss Prot
SynonymsK9; CK-9; EPPK; Cytokeratin 9 (KRT9)
Calculated MW62kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain
Cellular locationcytoskeleton, cytosol, extracellular exosome, extracellular space, nucleus.

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ABclonal:Western blot - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)}

Western blot - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)

Western blot analysis of lysates from Mouse brain, using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)}

Immunofluorescence - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)

Immunofluorescence analysis of paraffin-embedded human skin using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)}

Immunofluorescence - Cytokeratin 9 (KRT9) Rabbit pAb (A10119)

Immunofluorescence analysis of paraffin-embedded mouse skin using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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