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DCAF11 Rabbit pAb (A15519)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DCAF11 Rabbit pAb (A15519)

Western blot analysis of lysates from Mouse liver, using DCAF11 Rabbit pAb (A15519) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameDCAF11 Rabbit pAb
Catalog No.A15519
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human DCAF11 (NP_079506.3).
SequenceMGSRNSSSAGSGSGDPSEGLPRRGAGLRRSEEEEEEDEDVDLAQVLAYLLRRGQVRLVQGGGAANLQFIQALLDSEEENDRAWDGRLGDRYNPPVDATPDTRELEFNEIKTQVELATGQLGLRRAAQKHSFPRMLHQRERGLCHRGSFSLGEQSRVISHFLPNDLGFTDS
Gene ID
Swiss Prot
SynonymsGL014; WDR23; PRO2389; DCAF11
Calculated MW62kDa
Observed MW61kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver
Cellular locationnucleoplasm.
Customer validation

WB(Mus musculus, Homo sapiens, Mus musculus)

Documents

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    ABclonal:Western blot - DCAF11 Rabbit pAb (A15519)}

    Western blot - DCAF11 Rabbit pAb (A15519)

    Western blot analysis of lysates from Mouse liver, using DCAF11 Rabbit pAb (A15519) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15519? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)