제품 > 항체 > 다중클론항체(pAb)

DCTD Rabbit pAb (A5889)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DCTD Rabbit pAb (A5889)

Western blot analysis of various lysates using DCTD Rabbit pAb (A5889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - DCTD Rabbit pAb (A5889)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using DCTD Rabbit pAb (A5889) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DCTD Rabbit pAb (A5889)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DCTD Rabbit pAb (A5889) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameDCTD Rabbit pAb
Catalog No.A5889
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human DCTD (NP_001912.2).
SequenceMSEVSCKKRDDYLEWPEYFMAVAFLSAQRSKDPNSQVGACIVNSENKIVGIGYNGMPNGCSDDVLPWRRTAENKLDTKYPYVCHAELNAIMNKNSTDVKGCSMYVALFPCNECAKLIIQAGIKEVIFMSDKYHDSDEATAARLLFNMAGVTFRKFIPKCSKIVIDFDSINSRPSQKLQ
Gene ID
Swiss Prot
SynonymsDCTD
Calculated MW20kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSW620, Mouse heart, Rat heart
Cellular locationcytosol

Documents

Certificate of Compliance

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    ABclonal:Western blot - DCTD Rabbit pAb (A5889)}

    Western blot - DCTD Rabbit pAb (A5889)

    Western blot analysis of various lysates using DCTD Rabbit pAb (A5889) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - DCTD Rabbit pAb (A5889)}

    Immunohistochemistry - DCTD Rabbit pAb (A5889)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using DCTD Rabbit pAb (A5889) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - DCTD Rabbit pAb (A5889)}

    Immunohistochemistry - DCTD Rabbit pAb (A5889)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DCTD Rabbit pAb (A5889) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.