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DDX6 Rabbit pAb (A16270)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DDX6 Rabbit pAb (A16270)

Western blot analysis of various lysates using DDX6 Rabbit pAb (A16270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - DDX6 Rabbit pAb (A16270)

Immunohistochemistry analysis of paraffin-embedded rat brain using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DDX6 Rabbit pAb (A16270)

Immunohistochemistry analysis of paraffin-embedded human mammary cancer using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DDX6 Rabbit pAb (A16270)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - DDX6 Rabbit pAb (A16270)

Immunofluorescence analysis of L929 cells using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDDX6 Rabbit pAb
Catalog No.A16270
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 384-483aa of human DDX6 (NP_001244120.1).
SequenceLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP
Gene ID
Swiss Prot
SynonymsP54; RCK; HLR2; IDDILF; Rck/p54; DDX6
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, HeLa, NIH/3T3, Rat spleen
Cellular locationCytoplasm, P-body
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - DDX6 Rabbit pAb (A16270)}

Western blot - DDX6 Rabbit pAb (A16270)

Western blot analysis of various lysates using DDX6 Rabbit pAb (A16270) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - DDX6 Rabbit pAb (A16270)}

Immunohistochemistry - DDX6 Rabbit pAb (A16270)

Immunohistochemistry analysis of paraffin-embedded rat brain using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DDX6 Rabbit pAb (A16270)}

Immunohistochemistry - DDX6 Rabbit pAb (A16270)

Immunohistochemistry analysis of paraffin-embedded human mammary cancer using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DDX6 Rabbit pAb (A16270)}

Immunohistochemistry - DDX6 Rabbit pAb (A16270)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - DDX6 Rabbit pAb (A16270)}

Immunofluorescence - DDX6 Rabbit pAb (A16270)

Immunofluorescence analysis of L929 cells using DDX6 Rabbit pAb (A16270) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16270? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)