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DECR1 Rabbit pAb (A13014)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DECR1 Rabbit pAb (A13014)

Western blot analysis of various lysates using DECR1 Rabbit pAb (A13014) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): 293T.
Exposure time: 30s.

ABclonal:Western blot - DECR1 Rabbit pAb (A13014)

Western blot analysis of various lysates using DECR1 Rabbit pAb (A13014) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameDECR1 Rabbit pAb
Catalog No.A13014
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables 2, 4-dienoyl-CoA reductase (NADPH) activity; NADPH binding activity; and identical protein binding activity. Involved in fatty acid beta-oxidation. Located in cytosol; mitochondrion; and nucleoplasm. Part of catalytic complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 35-335 of human DECR1 (NP_001350.1).
SequenceNTEALQSKFFSPLQKAMLPPNSFQGKVAFITGGGTGLGKGMTTLLSSLGAQCVIASRKMDVLKATAEQISSQTGNKVHAIQCDVRDPDMVQNTVSELIKVAGHPNIVINNAAGNFISPTERLSPNAWKTITDIVLNGTAFVTLEIGKQLIKAQKGAAFLSITTIYAETGSGFVVPSASAKAGVEAMSKSLAAEWGKYGMRFNVIQPGPIKTKGAFSRLDPTGTFEKEMIGRIPCGRLGTVEELANLAAFLCSDYASWINGAVIKFDGGEEVLISGEFNDLRKVTKEQWDTIEELIRKTKGS
Gene ID
Swiss Prot
SynonymsDECR; NADPH; SDR18C1; DECR1
Calculated MW36kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSK-BR-3, Mouse liver, Mouse heart
Cellular locationMitochondrion.
Customer validation

WB(Homo sapiens, Rattus norvegicus)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - DECR1 Rabbit pAb (A13014)}

    Western blot - DECR1 Rabbit pAb (A13014)

    Western blot analysis of various lysates using DECR1 Rabbit pAb (A13014) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): 293T.
    Exposure time: 30s.
    ABclonal:Western blot - DECR1 Rabbit pAb (A13014)}

    Western blot - DECR1 Rabbit pAb (A13014)

    Western blot analysis of various lysates using DECR1 Rabbit pAb (A13014) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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