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DEFA3 Rabbit pAb (A5340)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - DEFA3 Rabbit pAb (A5340)

Western blot analysis of extracts of various cell lines, using DEFA3 antibody (A5340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameDEFA3 Rabbit pAb
Catalog No.A5340
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-94 of human DEFA3 (NP_005208.1).
SequenceEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMDCYCRIPACIAGERRYGTCIYQGRLWAFCC
Gene ID
Swiss Prot
SynonymsHP3; DEF3; HNP3; HP-3; HNP-3
Calculated MW10kDa
Observed MW13kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, 22Rv1, SW480
Cellular locationSecreted
    ABclonal:Western blot - DEFA3 Rabbit pAb (A5340)}

    Western blot - DEFA3 Rabbit pAb (A5340)

    Western blot analysis of extracts of various cell lines, using DEFA3 antibody (A5340) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.

    * For research use only. Not for therapeutic or diagnostic purposes.