제품 > 항체 > 다중클론항체(pAb)

DHDDS Rabbit pAb (A7835)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DHDDS Rabbit pAb (A7835)

Western blot analysis of various lysates using DHDDS Rabbit pAb (A7835) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameDHDDS Rabbit pAb
Catalog No.A7835
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several classes of glycoproteins. Mutations in this gene are associated with retinitis pigmentosa type 59. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human DHDDS (NP_995583.1).
SequenceIKAGPMPKHIAFIMDGNRRYAKKCQVERQEGHSQGFNKLAETLRWCLNLGILEVTVYAFSIENFKRSKSEVDGLMDLARQKFSRLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQATKNYNKCFLNVCFAYTSRHEISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHP
Gene ID
Swiss Prot
SynonymsDS; CIT; CPT; HDS; RP59; hCIT; DEDSM; DHDDS
Calculated MW39kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Mouse testis, Rat thymus
Cellular locationEndoplasmic reticulum membrane, Peripheral membrane protein.
    ABclonal:Western blot - DHDDS Rabbit pAb (A7835)}

    Western blot - DHDDS Rabbit pAb (A7835)

    Western blot analysis of various lysates using DHDDS Rabbit pAb (A7835) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.