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DIS3L Rabbit pAb (A16160)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DIS3L Rabbit pAb (A16160)

Western blot analysis of various lysates using DIS3L Rabbit pAb (A16160) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunohistochemistry - DIS3L Rabbit pAb (A16160)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using DIS3L Rabbit pAb (A16160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameDIS3L Rabbit pAb
Catalog No.A16160
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The cytoplasmic RNA exosome complex degrades unstable mRNAs and is involved in the regular turnover of other mRNAs. The protein encoded by this gene contains 3'-5' exoribonuclease activity and is a catalytic component of this complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DIS3L (NP_588616.1).
SequenceMQTACQAVQHQRGRRQYNKLRNLLKDARHDCILFANEFQQCCYLPRERGESMEKWQTRSIYNAAVWYYHHCQDRMPIVMVTEDEEAIQQYGSETEGVFVITFKNYLDNFWPDLKAAHELCDSILQSRRERENESQESHGKEYPEHLPLEVLEAGIKSGRYIQGILNVNKHRAQIEAFVRLQGASSKDSDLVSDILIHGMKARNRSIHGDVVVVELLPKNEWKGRTVALCENDCDDKASGESPSEPMPTGRVVGILQKNWRDYVVTFPSKEEVQSQGKNAQKILVTPWDYRIPKIRISTQQ
Gene ID
Swiss Prot
SynonymsDIS3L1; DIS3L
Calculated MW121kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, HeLa, Mouse testis, Rat thymus
Cellular locationCytoplasm

Documents

Certificate of Compliance

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    ABclonal:Western blot - DIS3L Rabbit pAb (A16160)}

    Western blot - DIS3L Rabbit pAb (A16160)

    Western blot analysis of various lysates using DIS3L Rabbit pAb (A16160) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.
    ABclonal:Immunohistochemistry - DIS3L Rabbit pAb (A16160)}

    Immunohistochemistry - DIS3L Rabbit pAb (A16160)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using DIS3L Rabbit pAb (A16160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.