제품 > 항체 > 다중클론항체(pAb)

DLDH/DLD Rabbit pAb (A13296)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using DLDH/DLD Rabbit pAb (A13296) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - DLDH/DLD Rabbit pAb (A13296)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using DLDH/DLDH/DLDH/DLD Rabbit pAb (A13296) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human placenta using DLDH/DLDH/DLDH/DLD Rabbit pAb (A13296) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - DLDH/DLD Rabbit pAb (A13296)

Immunofluorescence analysis of HeLa cells using DLDH/DLD Rabbit pAb (A13296). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	DLDH/DLD Rabbit pAb
Catalog No.	A13296
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLDH/DLDH/DLDH/DLD (NP_000099.2).
Sequence	ADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGK
Gene ID
Swiss Prot
Synonyms	E3; LAD; DLDD; DLDH; GCSL; PHE3; OGDC-E3; DLDH/DLD
Calculated MW	54kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	MCF7, BT-474, K-562, 293T, Mouse liver
Cellular location	Mitochondrion matrix
Customer validation	WB(Homo sapiens) IHC(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

DLDH/DLD Rabbit pAb images

Western blot - DLDH/DLD Rabbit pAb (A13296)

Immunohistochemistry - DLDH/DLD Rabbit pAb (A13296)

Immunofluorescence - DLDH/DLD Rabbit pAb (A13296)

Immunofluorescence analysis of HeLa cells using DLDH/DLD Rabbit pAb (A13296). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.