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DLL1 Rabbit pAb (A14277)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DLL1 Rabbit pAb (A14277)

Western blot analysis of lysates from Rat lung, using DLL1 Rabbit pAb (A14277) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - DLL1 Rabbit pAb (A14277)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using DLL1 Rabbit pAb (A14277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DLL1 Rabbit pAb (A14277)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using DLL1 Rabbit pAb (A14277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameDLL1 Rabbit pAb
Catalog No.A14277
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-220 of human DLL1 (NP_005609.3).
SequenceDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPY
Gene ID
Swiss Prot
SynonymsDL1; Delta; DELTA1; NEDBAS; DLL1
Calculated MW78kDa
Observed MW78kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat lung
Cellular locationApical cell membrane, Cell junction, Membrane raft, Single-pass type I membrane protein, adherens junction.

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ABclonal:Western blot - DLL1 Rabbit pAb (A14277)}

Western blot - DLL1 Rabbit pAb (A14277)

Western blot analysis of lysates from Rat lung, using DLL1 Rabbit pAb (A14277) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - DLL1 Rabbit pAb (A14277)}

Immunohistochemistry - DLL1 Rabbit pAb (A14277)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using DLL1 Rabbit pAb (A14277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - DLL1 Rabbit pAb (A14277)}

Immunohistochemistry - DLL1 Rabbit pAb (A14277)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using DLL1 Rabbit pAb (A14277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)