제품 > 항체 > 단일클론항체(mAb)

DNAJA4 Rabbit mAb (A21155)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DNAJA4 Rabbit mAb (A21155)

Western blot analysis of various lysates using DNAJA4 Rabbit mAb (A21155) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - DNAJA4 Rabbit mAb (A21155)

Western blot analysis of lysates from THP-1 cells, using DNAJA4 Rabbit mAb (A21155) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunoprecipitation - DNAJA4 Rabbit mAb (A21155)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg DNAJA4 antibody (A21155). Western blot was performed from the immunoprecipitate using DNAJA4 antibody (A21155) at a dilution of1:1000.

Overview

Product nameDNAJA4 Rabbit mAb
Catalog No.A21155
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3009
Enables chaperone binding activity and unfolded protein binding activity. Involved in several processes, including negative regulation of endothelial cell migration; negative regulation of inclusion body assembly; and protein refolding. Located in cytosol and membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-397 of human DNAJA4 (NP_057538.1).
SequenceDLRCVRDEGMPIYKAPLEKGILIIQFLVIFPEKHWLSLEKLPQLEALLPPRQKVRITDDMDQVELKEFCPNEQNWRQHREAYEEDEDGPQAGVQCQTA
Gene ID
Swiss Prot
SynonymsMST104; MSTP104; PRO1472; DNAJA4
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesMouse heart, Rat heart, Mouse brain
Cellular locationcytoplasm, cytosol

Documents

Certificate of Compliance

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ABclonal:Western blot - DNAJA4 Rabbit mAb (A21155)}

Western blot - DNAJA4 Rabbit mAb (A21155)

Western blot analysis of various lysates using DNAJA4 Rabbit mAb (A21155) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - DNAJA4 Rabbit mAb (A21155)}

Western blot - DNAJA4 Rabbit mAb (A21155)

Western blot analysis of lysates from THP-1 cells, using DNAJA4 Rabbit mAb (A21155) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunoprecipitation - DNAJA4 Rabbit mAb (A21155)}

Immunoprecipitation - DNAJA4 Rabbit mAb (A21155)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg DNAJA4 antibody (A21155). Western blot was performed from the immunoprecipitate using DNAJA4 antibody (A21155) at a dilution of1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.