제품 > 항체 > 다중클론항체(pAb)

DNAJC10 Rabbit pAb (A12143)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - DNAJC10 Rabbit pAb (A12143)

Western blot analysis of various lysates using DNAJC10 Rabbit pAb (A12143) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - DNAJC10 Rabbit pAb (A12143)

Immunofluorescence analysis of L929 cells using DNAJC10 Rabbit pAb (A12143) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDNAJC10 Rabbit pAb
Catalog No.A12143
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 614-793 of human DNAJC10 (NP_061854.1).
SequenceIDCQQYHSFCAQENVQRYPEIRFFPPKSNKAYHYHSYNGWNRDAYSLRIWGLGFLPQVSTDLTPQTFSEKVLQGKNHWVIDFYAPWCGPCQNFAPEFELLARMIKGKVKAGKVDCQAYAQTCQKAGIRAYPTVKFYFYERAKRNFQEEQINTRDAKAIAALISEKLETLRNQGKRNKDEL
Gene ID
Swiss Prot
SynonymsJPDI; MTHr; ERdj5; PDIA19; DNAJC10
Calculated MW91kDa
Observed MW91kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, HT-29, SKOV3, Raji, SGC-7901
Cellular locationEndoplasmic reticulum lumen

Documents

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    ABclonal:Western blot - DNAJC10 Rabbit pAb (A12143)}

    Western blot - DNAJC10 Rabbit pAb (A12143)

    Western blot analysis of various lysates using DNAJC10 Rabbit pAb (A12143) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - DNAJC10 Rabbit pAb (A12143)}

    Immunofluorescence - DNAJC10 Rabbit pAb (A12143)

    Immunofluorescence analysis of L929 cells using DNAJC10 Rabbit pAb (A12143) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.