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DNAJC17 Rabbit pAb (A20168)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DNAJC17 Rabbit pAb (A20168)

Western blot analysis of various lysates using DNAJC17 Rabbit pAb (A20168) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - DNAJC17 Rabbit pAb (A20168)

Western blot analysis of various lysates using DNAJC17 Rabbit pAb (A20168) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - DNAJC17 Rabbit pAb (A20168)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using DNAJC17 Rabbit pAb (A20168) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DNAJC17 Rabbit pAb (A20168)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using DNAJC17 Rabbit pAb (A20168) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameDNAJC17 Rabbit pAb
Catalog No.A20168
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable RNA binding activity. Predicted to act upstream of or within negative regulation of transcription by RNA polymerase II and toxin transport. Predicted to be located in cytoplasm and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human DNAJC17 (NP_060633.1).
SequenceMAVTKELLQMDLYALLGIEEKAADKEVKKAYRQKALSCHPDKNPDNPRAAELFHQLSQALEVLTDAAARAAYDKVRKAKKQAAERTQKLDEKRKKVKLDLEARERQAQAQESEEEEESRSTRTLEQEIERLREEGSRQLEEQQRLIREQIRQERDQRLRGKAENTEGQGTPKLKLKWKCKKEDESKGGYSKDVLLRLLQKYGEVLNLVLSSKKPGTAVVEFATVKAAELAVQNEVGLVDNPLKISWLEGQPQDAVGRSHSGLSKGSVLSERDYESLVMMRMRQAAERQQLIARMQQEDQEGPPT
Gene ID
Swiss Prot
SynonymsDNAJC17
Calculated MW35kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, A-549, Mouse testis, Mouse thymus, Rat testis
Cellular locationcytoplasm.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - DNAJC17 Rabbit pAb (A20168)}

    Western blot - DNAJC17 Rabbit pAb (A20168)

    Western blot analysis of various lysates using DNAJC17 Rabbit pAb (A20168) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - DNAJC17 Rabbit pAb (A20168)}

    Western blot - DNAJC17 Rabbit pAb (A20168)

    Western blot analysis of various lysates using DNAJC17 Rabbit pAb (A20168) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - DNAJC17 Rabbit pAb (A20168)}

    Immunohistochemistry - DNAJC17 Rabbit pAb (A20168)

    Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using DNAJC17 Rabbit pAb (A20168) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - DNAJC17 Rabbit pAb (A20168)}

    Immunohistochemistry - DNAJC17 Rabbit pAb (A20168)

    Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using DNAJC17 Rabbit pAb (A20168) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.