제품 > 항체 > 다중클론항체(pAb)

DNAJC18 Rabbit pAb (A17843)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - DNAJC18 Rabbit pAb (A17843)

Western blot analysis of various lysates using DNAJC18 Rabbit pAb (A17843) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 300s.

Overview

Product nameDNAJC18 Rabbit pAb
Catalog No.A17843
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable Hsp70 protein binding activity. Predicted to be involved in cellular response to misfolded protein; chaperone cofactor-dependent protein refolding; and ubiquitin-dependent ERAD pathway. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 260-350 of human DNAJC18 (NP_689899.1).
SequenceSTLGYTISRETQNLQVPYFVDKNFDKAYRGASLHDLEKTIEKDYIDYIQTSCWKEKQQKSELTNLAGLYRDERLKQKAESLKLENCEKLSK
Gene ID
Swiss Prot
SynonymsDNAJC18
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesBxPC-3, Jurkat
Cellular location

Documents

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    ABclonal:Western blot - DNAJC18 Rabbit pAb (A17843)}

    Western blot - DNAJC18 Rabbit pAb (A17843)

    Western blot analysis of various lysates using DNAJC18 Rabbit pAb (A17843) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 300s.

    * For research use only. Not for therapeutic or diagnostic purposes.