제품 > 항체 > 다중클론항체(pAb)

DNA Ligase IV Rabbit pAb (A1743)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit pAb (A1743) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - DNA Ligase IV Rabbit pAb (A1743)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DNA Ligase IV Rabbit pAb (A1743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	DNA Ligase IV Rabbit pAb
Catalog No.	A1743
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DNA Ligase IV (NP_002303.2).
Sequence	MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHND
Gene ID
Swiss Prot
Synonyms	LIG4S; DNA Ligase IV
Calculated MW	104kDa
Observed MW	110kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	PC-3
Cellular location	Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus)

Documents

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Research Area

DNA Ligase IV Rabbit pAb images

Western blot - DNA Ligase IV Rabbit pAb (A1743)

Immunohistochemistry - DNA Ligase IV Rabbit pAb (A1743)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Journal of Cell Science

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

29760279

Title:

Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability.

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Aging

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

29227966

Title:

POT1 inhibits the efficiency but promotes the fidelity of nonhomologous end joining at non-telomeric DNA regions

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Cell Death And Differentiation

Application:

IF:

13.7

Species:

Homo sapiens

PMID:

27391797

Title:

Impaired DNA double-strand break repair contributes to the age-associated rise of genomic instability in humans

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Oncotarget

Application:

IF:

Species:

Homo sapiens

PMID:

28030852

Title:

Drug-induced premature senescence model in human dental follicle stem cells.

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Aging Cell

Application:

IF:

Species:

Homo sapiens

PMID:

33455051

Title:

Lycorine hydrochloride suppresses stress‐induced premature cellular senescence by stabilizing the genome of human cells

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Science advances

Application:

IF:

11.7

Species:

Homo sapiens

PMID:

37315132

Title:

SMYD2 inhibition-mediated hypomethylation of Ku70 contributes to impaired nonhomologous end joining repair and antitumor immunity - PMC

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

EMBO reports

Application:

IF:

6.5

Species:

Homo sapiens

PMID:

37955230

Title:

Androgen signaling stabilizes genomes to counteract senescence by promoting XRCC4 transcription

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.1

Species:

Homo sapiens

PMID:

38238314

Title:

The P286R mutation of DNA polymerase ε activates cancer-cell-intrinsic immunity and suppresses endometrial tumorigenesis via the cGAS-STING pathway - PMC

References for DNA Ligase IV Rabbit pAb (A1743)

Product:

DNA Ligase IV Rabbit pAb

Journal:

Cell death and differentiation

Application:

IF:

13.7

Species:

Homo sapiens

PMID:

38664591